As infections from antibiotic-resistant bacteria increase the discovery of new antibiotic has halted. If left unchecked, this imbalance could result in over ten million deaths annually by 2050. To address this issue, extracts from soil samples were screened for antibacterial properties, and their efficacy and mechanisms of action were determined. This screen isolated compounds with bactericidal activity against B. anthracis, E. coli, and P. aeruginosa, which present promising findings for combating these deadly pathogens.