This pre-clinical study is aimed at examining the mechanism by which L-Citrulline may impact vascular activity and protein synthesis in a model of insulin-resistance. In L6 myotubes, L-Citrulline shows promising results of impacting protein synthesis through the mTOR pathway, as well as a potential effect on endothelial activity. Further research is needed to determine the exact function of L-Citrulline in a cellular model. This may eventually warrant translational research in Type 2 diabetic humans.