Lyme disease is the most common tick-borne disease in the US. Blacklegged ticks are the vectors for Borrelia burgdorferi, the Lyme disease pathogen. Eastern grey squirrels are one of many hosts for blacklegged ticks, but there is minimal research about the role of squirrels in tick ecology and Lyme disease epidemiology. The purpose of this study is to further understand the relationship between blacklegged ticks and Eastern grey squirrels.