The AggTag fluorogenic detection method involves chemically labeling a protein-of-interest (POI) with a fluorophore via a linker to visualize protein aggregates. The assumption that linker size interferes with protein folding was investigated via the synthesis of Halo tag (33 kDa) and Snap-tag (19 kDa) linkers, attachment to POI, and testing of the optical density at various temperatures. It was found that protein aggregates form at a lower temperature when labeled with SNAP-tag vs. Halo-tag.