Sigma 54 dependent transcription and transcription factors are important to study in the squid vibrio system because they play a vital role in symbiosis of the Hawaiian Bobtail Squid, Euprymna scolopes. Sigma 54 dependent transcription is important to study outside of the squid vibrio system because it controls important bacterial stress response genes in pathogenicity and in agriculture5. Here we show that sigma54 dependent transcription is not necessary for growth and bioluminescence in rich media.