Lipoproteins are abundant proteins found on the surface of cell membranes. Lipoproteins are very important ligands for detection by the innate immune system. The main goal of this research is to identify how any structural changes to lipoproteins may affect Toll-like Receptor detection. Detergent partitioning is used to extract and analyze lipoprotein structure in Bifidobactrerium angulatum. We are currently optimizing liporptein extraction protocols to improve yields from Bifido species.