Liquid-liquid phase separation (LLPS) is a phenomenon involving the formation of macromolecule-rich droplets, also known as coacervate droplets, formed from interactions between oppositely charged molecules suspended in a dilute phase. Coacervates composed of polypeptides and RNA are appealing as prebiotic compartments since these molecules can undergo LLPS in low concentrations. Here, I  present recent findings on the impact of pH, salinity, and different polypeptide concentrations on RNA’s ability to interact and accumulate in coacervate droplets.