PIP5K is a critical enzyme that is involved in the generation of PIP2 – a component critical to HGF-stimulated epithelial cell migration and integrin recycling. The PIP5K gene has various isozymes that include PIP5KA, PIP5KB, and PIP5KC. In hopes of determining which of these isozymes is most responsible for the synthesis of PIP2 during migration, I performed a series of gene knockouts and assays and tested the impact on integrin recycling and cellular migration.