Microtubules are essential for long-distance transport in neurons. Local microtubule production by nucleation particularly concentrates at dendritic branch points. To investigate why nucleation machinery concentrates at branch points, we used an RNAi knockdown approach to knock down nervous wreck (Nwk), a membrane curvature-recognizing protein, from Drosophila neurons. Nervous wreck knockdown showed no significant concentration change in clathrin nor gamma-tubulin, two proteins involved in nucleation, suggesting that nervous wreck is not essential for either protein’s localization.