The Danube River is one of the largest transnational rivers, crossing through ten countries and supporting the lives of 80 million people. This study attempted to assess the water quality (as specified by the EU Water Framework Directive) at five sampling sites within the Brăila Islands, Danube Basin by examining three types of aquatic organisms: phytoplankton, macrophytes, and macroinvertebrates. Qualitative analyses of these organisms were conducted to determine the ecological effects of human influence.