Knowledge about the structures of lipoproteins in the gut microbiome can provide important information on interactions with toll-like receptors, and innate immune system functionality. Bifidobacterium angulatum was identified to be lacking known genes causing acylation patterns such as LnsA, LnsB, Lnt, and Lit. This made Bifido angulatum a strong candidate for extraction of lipoproteins, followed by mass spectrometry for identification of structure and acylation pattern.