The Biological Function of Twister Ribozymes at a Splice Junction in Danio rerio (Zebrafish)

Benjamin Chang

Twister ribozymes are a class of small self-cleaving RNA with unknown biological functions that are conserved across many organisms. I identified two examples at a splice junction where they can influence transcript maturation and their adjacent nucleotides can provide regulation for self-cleavage activity. Analysis of the self-cleavage rates of eight constructs with differing amounts of flanking sequences suggests a difference in activity between the spliced and unspliced versions.

Major: 
Biochemistry and Molecular Biology
Exhibition Category: 
Physical Sciences
Exhibition Format: 
Poster Presentation
Campus: 
University Park
Faculty Sponsor: 
Philip Bevilacqua
Poster Number: 
50509