Lipoproteins are bound to the cell membrane through an acylated N-terminal cysteine residue and are present in most bacteria; yet, the structure is unknown. Mammalian innate immune system recognizes lipoproteins for infection defense. Toll-like receptor 2 (TLR2) recognizes the acylation pattern. Clostridium coccoides was isolated, confirmed by 16S rRNA Sanger sequencing, and used to extract lipoproteins through detergent partitioning. The acylation pattern could be characterized and tested how it influences the pro-inflammatory TLR2 signaling cascade.