CRISPR/Cas-9 as a functional technique to create adss-1 mutants

ADSS mutations in humans are predicted to be involved in nemalinemyopathy this mutation is attributed to a point mutation in the ADSS gene. Ingeneral, the study of different human mutations has been done with thereplication of said mutations in model organisms such as Caenorhabditiselegans. The usual chosen technique used is RNAi given that it has a low costand is easier to use than other genetic techniques. Our goal with this experimentis to use the genetic editing technique CRISPR/Cas-9 in order to create a mutantwith an ADSS knock down function which we can use for further study andcombine different techniques in the future such as RNAi.