Axon injury induces an injury response pathway in Drosophila neurons which is accompanied by an increase in microtubule dynamics and production of microtubule severing protein, spastin. These experiments not only utilize the mechanisms behind this response pathway, but aim to understand them. We use an axon injury assay to determine the location to microtubule nucleation in axons. Additionally, we measure the effect of spastin on microtubule dynamics to elucidate it's role in the injury pathway.