We approached the problem of generating effective scar-preventing wound healing therapies that restore normal matrix structure and full function of tissue by optimizing a 3D microtissue wound healing model. To analyze the wound healing behavior, we administered 3 types of wounds on the microtissues: physical, chemical, and thermal. We observed cell proliferation, migration, tissue regeneration, contractility, and wound closure rates. We found that the features of this model coincided with the wound-healing process.