Inserting exogenous DNA into the genome of a cell using CRISPR/Cas-9 is highly inefficient. I hypothesize that the efficiency of this process can be improved by perturbing the cell cycle or reducing nonhomologous end joining activity. I designed and used a knock-in reporter that generates a fluorescent protein capable of detecting DNA insertion events. This system will allow us to identify those conditions that increase incorporation efficiency and assist in developing knock-in cell lines.