The role of ligand-activated nuclear receptor, peroxisome proliferator-activated receptor beta (PPARb), in cancer is not completely understood. Since PPARs regulate genes involved in metabolism, metabolic changes in PPARb loss of function and gain of function cancer cells were assessed using liquid chromatography - mass spectroscopy. Results suggest that PPARb modulates glycolysis which could relate to its decreased tumorigenic phenotype. Establishing this connection could lead to progress in colon cancer treatment options.