Yash Dixit
Ethylene Forming Enzyme (EFE) is a iron (II) and 2-oxoglutarate dependent enzyme that can produce the compound ethylene as well as trace amounts of 3-hydroxypropionate, a valuable chemical building block with a variety of applications including the synthesis of biodegradable plastics. Our project seeks to perform protein engineering of EFE by amino acid substitutions in the substrate binding pocket such that 3HP is the primary enzymatic product, and we present recent results of such substitutions.