Streptococcus mitis is an opportunistic pathogen that is resistant to common antibiotics. The Keiler Lab has identified 46 small molecule inhibitors that block the trans-translation pathway in bacteria. KKL-35, a small molecule inhibitor, was found to be active at 2.5 µM in a S. mitis culture. The biological target of the oxadiazol class of small molecule inhibitors can be determined in S. mitis by utilizing a click reaction with a similarly structured probe.