Stopped-Flow Kinetics of the Folding of Fluorescent RNA Sequences Under In Vivo-like Conditions

Kelley Melanson

Analyzing the kinetics of RNA folding can facilitate an understanding of the differences between thermodynamically predicted structures and those formed within the cell. Therefore, it is relevant to study the kinetics of RNA folding and the influencing factors. Consequently, a stopped-flow was used to record fluorescence of RNA duplex formation over millisecond intervals of time as a function of concentration and sequence in different solutions, allowing values of kobs to be obtained and analyzed.   

Major: 
Chemistry
Exhibition Category: 
Physical Sciences
Exhibition Format: 
Poster Presentation
Campus: 
University Park
Faculty Sponsor: 
Philip Bevilacqua
Poster Number: 
50496