Mitochondria package their genomes into membraneless nucleoprotein complexes called mt-nucleoids that serve as sites of replication. Here, we study how the key packaging protein mtSSB drives the condensation of single stranded DNA (ssDNA). Using PCR techniques, we generate templates of ssDNA, and we visualize the co-phase behavior of mtSSB and ssDNA using advanced light microscopy techniques. These results will shed light on the biophysical driving forces needed to organize replicating strands of DNA within mt-nucleoids.