Streptococcus mitis is an opportunistic pathogen that often causes bacteremia in cancer patients. This project aims to characterize and identify lipoproteins present in the membrane of S. mitis by growing S. mitis from stock and running PCR to confirm its presence, enriching lipoproteins, and separating them out using SDS-PAGE before using mass spectrometry to characterize and identify them. Lipoproteins have many functions, making it a potential target for new drug classes.