As we age, decreasing levels of NAD+ (Nicotinamide Adenine Dinucleotide) can lead to age-related complications such as acute kidney injury (AKI). Onset of AKI can lead to chronic kidney disease, kidney failure, and death. To tackle this relationship, we induced AKI in mouse models to investigate NAD+ biology. We quantified metabolic shifts using LCMS and mapped the expression of genes using qRT-PCR. Investigating AKI and dysregulation of NAD+ provided valuable insights into disease susceptibility.