This open-source acoustic plethysmograph generates thermal imaging of a mouse’s breath and volumetric data of the respiration volume of the mouse inside. This plethysmograph can also be used with a 2-photon microscope to generate neural imaging of the mouse while inside the vessel. The whole device is controlled through LabView and allows for user input to change the inner volume of the vessel or the outputting frequency to adjust the resulting resonance frequency.