A study was done to characterize both the phenotype and genotype of 36 Bacillus isolates. These isolates were obtained from mixed culture samples of clinical Bovine mastitis milk. Next-generation sequencing revealed that B.licheniformis encoded for the lipopeptide lichenysin A and the synthetase genes lchAA, lchAB, and lchAC for lichenysin. Preliminary observations suggest that B. licheniformis is an environmental mastitis pathogen, warranting further investigation into its potential to cause intramammary infections.