Yash Satyendra Dixit
Ethylene Forming Enzyme (EFE) is a iron (II) and 2-oxoglutarate dependent enzyme that can produce the compound ethylene as well as trace amounts of 3-hydroxypropionate, a valuable chemical building block with potential to synthesize biodegradable plastics. Our project seeks to perform protein engineering of EFE by amino acid substitutions in the substrate binding pocket such that 3HP is the primary enzymatic product, and also modify the 2-oxoglutarate substrate to synthesize substituted alkenes through the enzyme.