Symbiotic bacteria promote the health of an animal by performing a task that contributes toward their survival. A main determinant of symbiosis establishment is the physiology of the animal host, but environmental pressures experienced by an animal before or at reproductive age can lead to physiological abnormalities in offspring. The impact of such physiological abnormalities on an animal's ability to establish and maintain symbioses with bacterial symbionts is not fully understood, yet, essential for understanding the impact of environmental stressors on the future fitness of a species. The symbiosis between the Hawaiian Bobtail Squid Euprymna scolopes and the bioluminescent marine bacterium Vibrio fischeri presents a robust model for these investigations because the squid contains an organ that is physiologically selective for colonization by V. fischeri and symbiosis can be measured by the bioluminescence production of a squid. The main goal of this work is to identify the effect of environmental stress experienced by E. scolopes on the ability of subsequent generations of offspring to establish and maintain symbiosis with V. fischeri.