Extracellular signals and other cellular cargo are required for cell survival. Mechanisms of cargo internalization are needed in order to uptake these materials. Although events upstream and downstream of cargo intake in axons and the cell body of neurons are known, these events are not understood as well in dendrites. Using genetic manipulation in Drosophila and confocal microscopy techniques, we find that sites of endocytosis concentrates at dendritic branch points and act upstream of microtubule nucleation machinery recruitment, introducing a new importance of cell surroundings in neuron injury responses.