The importance of microtubules in neurons lies in their role of providing a highway network that is used to carry molecular components to various locations within the cell. Microtubule-severing proteins are responsible for regulating these microtubules to tailor the highway network to a cell’s needs. Kat60L1, one such severing protein, is being examined using in vivo live imaging in a Drosophila system to gain more insight into the specific functions of this poorly studied protein.