Transformation is the process of cells incorporating exogenous DNA into their genome; transformation efficiency denotes transformants from total viable cells. Efficiencies vary by species and plasmid, with difficulty in delineating consistently successful methodology. In Vibrio fischeri, transformation universally requires regulatory gene TfoX, but efficiencies are unconserved across isolates. The influential factors and methods to maximize transformation efficiency are poorly understood; gaining insight provides opportunity for increased genome editing consistency and a tool for mutant-comparative research.