Some long non-coding RNAs (lncRNAs) contain structural elements associated with their functions. RiboSNitches, which are single nucleotide polymorphisms (SNPs) that disrupt RNA secondary structures, may therefore affect their function. A genome-wide analysis was performed to test for purifying selection in 18,777 human lncRNAs. This involved three computational steps: predicting riboSNitches, identifying structured regions, and assessing riboSNitch depletion within them. We found that most lncRNAs contained few, if any, short structured regions.