Our lab studies how allergic asthma impacts internalizing disorders. To verify altered lung function in an experimental mouse model of allergic asthma, many labs use qPCR to quantify gene expression, however, there is no strong agreement on the best primers to use. We identified the best primers to measure interleukin-4, interleukin-5, and interleukin-13 gene expression in mouse lungs, using NIH databases and published research. Primers were confirmed using qPCR and gel electrophoresis.