Escherichia coli combats polymyxin B exposure by modifying lipopolysaccharide in the outer membrane. The arn operon is one of these pathways. Surrounding this operon is Insertion Sequence 1-18 (IS1-18). IS1-18 is able to produce a transposase when a -1 ribosomal frameshift occurs during translation. This can then duplicate IS1-18 and the operon. Aminoglycosides are antibiotics that bind to the ribosome, halting translation. Here, the effect of aminoglycosides on IS1-18-mediated polymyxin B resistance is investigated.