Cassiopea xamachana, commonly known as the upside-down jellyfish, maintains an obligate symbiosis with photosynthetic zooxanthellae. In this experiment, microbes from one jellyfish in Key Largo, Florida, are identified using environmental DNA extraction and PCR to amplify extracted DNA, then Oxford Nanopore MinION to sequence DNA. Data was processed and analyzed, and species were identified to match microbes identified from Cassiopea and other Cnidarians in existing literature, specifically with families Endozoicomonadaceae, Flavobacteriaceae, Rhodobacteraceae, and Litoricolaceae. ?