RNA thermometers (RNATs) regulate gene expression by sequestering the ribosome binding site at low temperatures and exposing it at higher temperatures. Work by the Bevilacqua lab discovered two novel RNATs in Bacillus subtilis that import glycerol (G) and glycerol-3-phosphate (G3P) at high temperatures. Using UV melts, we will test whether these imported solutes stabilize RNA structure. The purine riboswitch (xpt) from Bacillus subtilis has a known secondary structure and will be used for these studies.