Oxidative stress impact on Kinesin KIF1A structure and function.

Adrien Chen

Kinesin KIF1A, is a crucial motor protein that facilitates intra-cellular transport in neurons. We hypothesized that oxidative stress, present in neurodegenerative diseases, decreases kinesin's motility. Using TIRF microscopy, we found that H2O2 concentrations of 1 mM and higher decrease the motility of KIF1A, which could be rescued using Beta-mercaptoethanol. SDS-PAGE and Mass Spectrometry confirmed the presence of disulfide bonds between two monomeric heads. Our results suggest that KIF1A can be impacted by oxidative stress.

Major: 
Chemistry
Exhibition Category: 
Health and Life Sciences
Exhibition Format: 
Poster Presentation
Campus: 
University Park
Faculty Sponsor: 
William Hancock
Poster Number: 
29