Adrien Chen
Kinesin KIF1A, is a crucial motor protein that facilitates intra-cellular transport in neurons. We hypothesized that oxidative stress, present in neurodegenerative diseases, decreases kinesin's motility. Using TIRF microscopy, we found that H2O2 concentrations of 1 mM and higher decrease the motility of KIF1A, which could be rescued using Beta-mercaptoethanol. SDS-PAGE and Mass Spectrometry confirmed the presence of disulfide bonds between two monomeric heads. Our results suggest that KIF1A can be impacted by oxidative stress.