Stress may alter gene expression in brain regions linked to emotional regulation. This study examined whether adolescent restraint stress affects SOCS3 gene expression in the ventral hippocampus and prefrontal cortex of C57BL/6J male and female mice. RNA extraction, cDNA synthesis, and qPCR were conducted on brain tissue samples. Results showed no significant changes in SOCS3 expression. These findings suggest that stress does not impact SOCS3 gene expression in these brain regions.