Nipah virus (NiV), is a lethal zoonotic enveloped virus with increasing pandemic concern. Enveloped viruses use host factors like angiomotin-like 1 (AmotL1) to facilitate viral assembly and budding. We investigated the interaction between angiomotins and the NiV fusion (F) protein using the TurboID proximity labeling technique. We constructed a fused TurboID-AmotL1 construct and co-expressed it with the NiV F protein. We confirmed NiV F was in close proximity to AmotL1, as evidenced by its biotinylation.