E. coli is a commensal bacterium found in poultry, when given the opportunity it can cause colibacillosis infection. Sequencing genomes of colibacillosis from infected poultry is suspected to show different genes compared to commensal E. coli found in non-infected poultry. From sequencing analysis, these are differentiated by identification of possible virulence factor genes. Differentiation of colibacillosis-infected poultry and non-infected poultry can lead to further studies on prevention and treatment.